P-195: The Relationship between PAI-1 Polymorphism with Recurrent Implantation Failure (RIF)

Background: RIF is the most common cause of unsuccessful pregnancy after IVF. Among the various causes of RIF, the role of maternal genetic factors is of great significance. PAI-1 polymorphism is one of the notable polymorphisms in this field. In this study, the relationship between this polymorphism and the recurrent spontaneous abortions and infertility in different populations has been investigated. In other words, the relationship between this polymorphism and the occurrence of RIF has been investigated because there have been no studies on the patients suffering from New definitions of RIF in Iran. Materials and Methods: This descriptive-analytical study was conducted in Montaserieh Research and Clinical Center for Infertility of Mashhad University of Medical Sciences. It was carried out on 80 infertile couples after IVF from 2006 to 2011. Based on the new definitions of RIF, participants were divided into three groups: 1. Control group: Forty participants whose transferred embryos were successfully implanted. The selection criterion was the observation of gestational sac in ultrasonography twenty days after IVF 2. Patient group RIF 1: Those participants who received IVF two times and six embryos. In this group, no sign of pregnancy or forming gestational sac was found. 3. Patient group RIF 2: Those participants who received IVF at least three times and ten embryos. Again no sign of pregnancy and gestational sac was found. After receiving 5cc blood containing EDTA from participants, the process of DNA extraction was performed. Genotype of PAI-1 gene was determined by using PCR-ARMS technique. Gained frequencies in different groups were compared with each other using chi-square statistical analysis. Results: The frequency of 4G/5G genotype in control group, total participants, RIF1 group and RIF2 was 75,87, 80 and 95%, respectively. The frequency of 5G/5G genotype in control group, total participants patient group RIF 1, and patient group RIF 2 was orderly 25, 5, 5 and 5%. 4G/4G genotype was observed in none of the control groups and patient group RIF 1, But the observed 15% of patient RIF 2. Conclusion: According to our results, 4G/4G Polymorphism of PAI-1, is seen just in the second group of the patient (RIF 2), with the new definition of recurrent implantation failure. Hypofibrinolysis as a result of the 4G allele of the PAI-1 gene appears to be a risk factor for implantation failure by limiting trophoblastic invasion. So, with increasing number of IVF failure role of genetic factors become more significant.